Protein Labeling Reagents
Biotium CD32(7.3), CF568 conjugate, 0.1mg/mL
This MAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.Primary antibodies are available purified, or with a selection of fluor
Biotium CD48(156-4H9), CF568 conjugate, 0.1mg/mL
Reacts with human CD48, a 45 kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium CD36(1E8), CF568 conjugate, 0.1mg/mL
Recognizes a protein of 80 kDa-90 kDa, identified as CD36. It is expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV and V, P. falciparum malaria-infected erythrocytes, and sickle erythrocytes. It also functions as a scavenger receptor, mediating macrophage uptake of oxidized low-density lipoprotein (LDL) and recognition of apoptotic polymorphonuclear leukocytes (PMN). CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria. Note that 1-4% of Japanese and East Asia population lack CD36.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue
Biotium CD13(WM15), CF568 conjugate, 0.1mg/mL
Recognizes an integral membrane glycoprotein of 150 kDa, identified as CD13 (also known as aminopeptidase-N). The antibody recognizes an extracellular epitope. The CD13 antigen is present on most cells of myeloid origin including granulocytes, monocytes, mast cells, and GM-progenitor cells. It is also expressed by the majority of AML, CML in myeloid blast crisis, and in a smaller fraction of lymphoid leukemias. CD13 is absent from normal lymphocytes, platelets and erythrocytes. CD13 is also present on fibroblasts; endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 is identical to aminopeptidase N (APN), a prominent membrane-bound metalloprotease present on the surface of intestinal brush border and renal tubules. CD13 plays a role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal/tumoricidal activities. It also serves
Biotium CF770R Succinimidyl Ester
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red/near-IR fluorescent CF770R dye has excitation/emission at 770/701 nm.
Biotium Tyramide Amplification Kit with HRP Streptavidin and CF488A Tyramide
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. The kit contains HRP Streptavidin CF488A dye tyramide. The dye has an excitation/emission of 490/515 nm. The kit contains sufficient reagent for 50-150 slides.
Biotium Fluo-3, AM Ester, Special Packag, 20x50ug
Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening. Properties: Orange red solid soluble in DMSO; Store -20°C and protect from light, especially when in solution; C51H50Cl2N2O23; MW: 1129.9; [121714-22-5]
![eMolecules 2183472-97-9 | BDP 581/591 amine | Broadpharm526.860 | C28H34BClF2N4O | 98.000 | Cl.NCCCCCCNC(=O)CCc1ccc2C=C3C=CC(\C=C/C=C/c4ccccc4)=[N+]3[B-](F)(F)n12 | 50mg | 374905454](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502203478.JPG-250.jpg)
eMolecules 2183472-97-9 | BDP 581/591 amine | Broadpharm526.860 | C28H34BClF2N4O | 98.000 | Cl.NCCCCCCNC(=O)CCc1ccc2C=C3C=CC(\C=C/C=C/c4ccccc4)=[N+]3[B-](F)(F)n12 | 50mg | 374905454
BDP 581/591 amine | Broadpharm | 2183472-97-9526.860 | C28H34BClF2N4O | 98.000 | Cl.NCCCCCCNC(=O)CCc1ccc2C=C3C=CC(\C=C/C=C/c4ccccc4)=[N+]3[B-](F)(F)n12 | 50mg | 374905454
Sigma Aldrich Fine Chemicals Biosciences Fluorescein isothiocyanate isomer I suitable for protein labeling, >=90% (HPLC), powder | 3326-32-7 | MFCD00005063 | 250MG
Fluorescein isothiocyanate isomer I suitable for protein labeling, >=90% (HPLC), powder | Purity: >=90% (HPLC) | Mol Wt: 389.38 | 3326-32-7 | MFCD00005063 | 250MG
Sigma Aldrich Fine Chemicals Biosciences CyR3 Mono NHS Ester Cytiva
The packs contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups for example on antibodies peptides or oligonucleotides.
![eMolecules BP Fluor 546 Azide | Broadpharm |944.100 | C42H53N7O12S3 | 0.000 | CC1CC(C)(C)Nc2c1cc1C(c3ccc(cc3C(=O)N(C)CCS(O)(=O)=O)C(=O)NCCCCCCN=[N+]=[N-])=c3cc4C(C)CC(C)(C)N=c4c(c3Oc1c2S(O)(=O)=O)S(O)(=O)=O | 25mg | 599128640](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502183654.JPG-250.jpg)
eMolecules BP Fluor 546 Azide | Broadpharm |944.100 | C42H53N7O12S3 | 0.000 | CC1CC(C)(C)Nc2c1cc1C(c3ccc(cc3C(=O)N(C)CCS(O)(=O)=O)C(=O)NCCCCCCN=[N+]=[N-])=c3cc4C(C)CC(C)(C)N=c4c(c3Oc1c2S(O)(=O)=O)S(O)(=O)=O | 25mg | 599128640
BP Fluor 546 Azide | Broadpharm |944.100 | C42H53N7O12S3 | 0.000 | CC1CC(C)(C)Nc2c1cc1C(c3ccc(cc3C(=O)N(C)CCS(O)(=O)=O)C(=O)NCCCCCCN=[N+]=[N-])=c3cc4C(C)CC(C)(C)N=c4c(c3Oc1c2S(O)(=O)=O)S(O)(=O)=O | 25mg | 599128640
Cayman Chemical AM694 3-Iodo Isomer 5mg
An analog of AM694; intended for forensic and research applications
STEMCELL Technologies LysoBrite™ Lysosome Dye, Green, Size: 500 Tests
LysoBrite™ dyes are fluorescent dyes that selectively stain lysosomes and other acidic organelles. Due to their hydrophobic nature, LysoBrite™ dyes permeate live cells and selectively accumulate in the lysosomes via the lysosome’s pH gradient. The fluorescence property of these dyes significantly increases within the lysosome’s acidic environment; this fluorescence can be measured using flow cytometry and fluorescence imaging. Due to their selective fluorescence inside lysosomes, LysoBrite™ dyes provide low background and high contrast during imaging. They can be used with proliferating and non-proliferating cells, and are suitable for suspension and adherent cells.
Abcam Metho x y- x 04, amyloid beta fluorescent marker, 5MG
MW 344.4 Da, Purity >98%. Blood-brain barrier permeable amyloid β fluorescent marker. Blue fluorophore. Derivative of Congo Red (ab145645) and Chrysamine G. Selectively binds fibrillar β-sheet deposits with high affinity (Ki = 26.8 nM). Detects plaques, tangles and cerebrovascular amyloid in vivo. .
The product is subject to the following: Abcam Restricted Use Statement
Abcam 5(6)-Carbo x y- x -rhodamine, 25MG
MW 1069.2 Da, Purity >90%. Stable, long wavelength, water soluble fluorophore has found widespread use in DNA-labeling, cell staining and protein labeling applications.
The product is subject to the following: Abcam Restricted Use Statement